Table 3.

Results of Cerebrospinal Fluid Analysis

	Monkey ID
	5M02		5M04		5M05		5M01		5M03
	Dose MV-CEA (TCID50)
	0		2 ×105		2 × 105		2 × 106		2 × 106
CSF parameter	Day 9	One year	Day 9	One year	Day 9	One year	Day 9	One year	Day 9	One year
Glucose (mg/dl)	41	47	39	44	37	45	44	50	49	47
Total protein (mg/dl)	16	16	18	20	24	32	19	18	26	39
CEAa (ng/ml)	<0.5	<0.1	<0.5	<0.1	<0.5	<0.1	<0.5	<0.1	<0.5	<0.1
Anti-MVAb ELISAb (OD)	0.00	0.00	0.00	0	0.01	0.02	0.07	0.00	0.10	0.04
Total nucleated cell count (no./μl)	4	2	ND	7	ND	<1.0	59	3	ND	3
Erythrocytes (no./μl)	6	792	ND	0	ND	131	94	122	ND	32
CSF differential
Neutrophils (%)		6	7		4	11		7		2
Lymphocytes (%)	93	78	59	47	80	69	66	57	84	54
Monos/macros (%)	5	9	33	51	14	15	23	29	16	42
Eosinophils (%)	2	3	1	2	2	5	11	7		2
Other cells (%)		4
Comment (%)	c	c	c	c	c	c	c	c	c	c
Vero cell overlay (Pos/Neg)	Neg	Neg	Neg	Neg	Neg	Neg	Neg	Neg	Neg	Neg
QRT-PCR for MV-N gene (copies)	BLD		BLD		BLD		BLD		BLD

Abbreviations: BLD, below limit of detection for assay (<1000 copies/μg RNA); CEA, carcinoembryonic antigen; CSF, cerebrospinal fluid; ELISA, enzyme-linked immunosorbent assay; ID, identification; monos/macros, monocytes/macrophages; MV-CEA, measles virus derivative producing carcinoembryonic antigen; ND, not data (laboratory did not obtain counts before Cytospin of sample); OD, opical density; QRT-PCR, quantitative real-time polymerase chain reaction; TCID₅₀, median tissue culture infective dose.

^aAssay limit of detection improved from 0.5 to 0.1 ng/ml between sampling dates.

^bOD > 0.25 is positive.

^cNo blasts or malignant cells seen.