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. Author manuscript; available in PMC: 2010 Mar 1.
Published in final edited form as: Nat Struct Mol Biol. 2009 Aug 23;16(9):938–944. doi: 10.1038/nsmb.1650

Figure 3. FRET analyses of site exposure and decompaction for nucleosome arrays in 1 mM Mg2+.

Figure 3

(a) LexA titration of compaction-sensitive nucleosome arrays (DNA mp33) in 1 mM Mg2+, monitored by FRET. (b) As in (a) except using site exposure-sensitive arrays (DNA mp31). (c) Quantitative analysis of titrations from (a,b) and additional systems: compaction-sensitive arrays lacking a LexA site (mp34), and site exposure-sensitive mononucleosomes having (mp11) or lacking (mp12) a LexA site. (d) Apparent binding affinities (dissociation constants) of LexA from measurements such as those in (c). Error bars, standard deviations, n = 2-3.