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. Author manuscript; available in PMC: 2009 Sep 22.
Published in final edited form as: J Am Chem Soc. 2009 Feb 18;131(6):2224–2230. doi: 10.1021/ja807609m

Table 5.

EC50 Values and Specificity Ratios of Peptides Determined by Using a Solid-Phase Pull Assay Method in the Presence and Absence of tRNAmix a

peptide EC50 (nM) wo/tRNAmix EC50 (nM) w/tRNAmix specificity ratio
biotin-3d 100 ± 12 140 ± 38 1.4
biotin-1h 660 ± 310 1700 ± 210 2.6
a

For a solid-phase pull down assay, all peptides were biotinylated. 500 pM of IRES RNA was mixed w/5 nM of tRNAmix (10 equiv) as a competitor. Specificity Ratio (SR) = EC50 w/tRNAmix/EC50 wo/tRNAmix. Each value indicates the average and 1 SD of more than three independent experiments.