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. Author manuscript; available in PMC: 2009 Sep 22.
Published in final edited form as: Nat Struct Mol Biol. 2007 Dec 23;15(1):57–64. doi: 10.1038/nsmb1351

Table 1.

Translation initiation efficiency of mutant CrPV IRES RNAs

Mutant Translation
efficiencya
(F/R ratio)		 Translation
efficiencya
(% wild-type IRES)
WT 0.211 ± 0.038 100 ± 17.8
ΔPK1/ΔPKIIIb 0.009 ± 0.001 4 ± 1
U6185A 0.103 ± 0.018 49 ± 8.6
U6185C 0.040 ± 0.017 19 ± 8.2
U6185G 0.082 ± 0.005 39 ± 2.6
U6186A 0.044 ± 0.014 21 ± 6.4
U6186C 0.103 ± 0.019 49 ± 9.0
U6186G 0.068 ± 0.007 32 ± 3.4
G6192A 0.098 ± 0.015 47 ± 7.0
G6192C 0.025 ± 0.006 12 ± 2.7
Triple (U6185C + U6186A + G6192C) 0.0032 ± 0.0004 1.5 ± 0.2
Δ6191 0.315 ± 0.033 149 ± 16
6190–6191_comp 0.0032 ± 0.0004 1.5 ± 0.2
a

Average values and standard errors from at least three experiments are reported.

b

Mutant originally characterized in ref.13.