Table 2.
Data collection, phasing and refinement statisticsa
| Cobalt crystal | Iridium crystal | ||
|---|---|---|---|
| Data collection | |||
| Space group | P41212 | P41212 | |
| Cell dimensions | |||
| a, b, c (Å) | 58.58, 58.58, | 58.69, 58.69, | |
| 98.90 | 99.00 | ||
| Inflection | Peak | ||
| Wavelength | 1.5418 (Cu-Kα) | 1.1055 | 1.1053 |
| Resolution (Å) | 50.00–3.00 | 23.2–2.40 | 23.2–2.40 |
| Rmerge | 11.8 (53.8) | 9.8 | 9.0 |
| I / σI | 21.8 (4.5) | 27.9 (2.3) | 27.2 (2.0) |
| Completeness (%) | 99.8 (100) | 99.7 (99.8) | 99.7 (99.7) |
| Redundancy | 14.8 (14.9) | 31.7 (18.1) | 31.9 (18.3) |
| Refinement | |||
| Resolution (Å) | 50–2.4 | ||
| No. reflections (unique) | 7,225 | ||
| Rwork / Rfree | 22.9% / 26.0% | ||
| No. atoms | 1,106 | ||
| Nucleic acid | 932 | ||
| Ligand/ion | 28 | ||
| Water | 146 | ||
| B-factors | |||
| Nucleic acid | 44.17 | ||
| Ligand/ion | 62.0 | ||
| Water | 43.7 | ||
| R.m.s deviations | |||
| Bond lengths (Å ) | 0.005316 | ||
| Bond angles (°) | 1.35 | ||
a
Data were collected from two crystals; no native crystals were used. Values in parentheses are for highest resolution shell.