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. Author manuscript; available in PMC: 2010 Sep 15.
Published in final edited form as: Anal Chem. 2009 Sep 15;81(18):7823–7828. doi: 10.1021/ac901176t

Figure 2.

Figure 2

Identification of α-synuclein peptide E-35-GVLYNO2VGSKTK-45 containing 3NT modification at Tyr-39. Human α-synuclein was in vitro nitrated, proteolytically digested with Asp-N and then analyzed by MS/MS. A) MS/MS spectra of unmodified human α-synuclein peptide (Asp-N digested) containing Tyr-39, E-35-GVLYVGSKTK-45 at m/z 394.2, (M=1179.6 Da). B) Corresponding MS/MS spectra of the peptide containing 3NT, E-35-GVLYNO2VGSKTK-45 (YNO2 = 3NT) at m/z 409.2 (M=1224.5 Da). Note the low mass immonium (IMM) ions (+NH2=CH-R), for Tyr and 3NT at m/z 136.1 and 181.1, respectively.