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. Author manuscript; available in PMC: 2009 Sep 22.
Published in final edited form as: Nature. 2007 Oct 18;449(7164):919–922. doi: 10.1038/nature06205

Figure 3. IFNβ-induced miRNAs directly target viral genomic RNA.

Figure 3

a, b, An infectious chimaeric virus was constructed from JFH1 and J6CF as shown in a; the numbers in brackets indicate the location in the virion (nucleotides) that the miRNA binds. The Huh7-cell subclone Huh7.5.1c2 was transfected with either miR-196 or miR-448, or with the mutant miR-196* and miR-448* harbouring a compensatory mutation in the seed sequence as outlined in a. Transfected Huh7.5.1c2 cells were infected with JFH1(D183) (ref. 23) or chimaeric J6/JFH, and HCV RNA was quantified by qPCR after 24 or 60 h post infection, respectively, during the phase of exponential viral RNA amplification, to accommodate the difference in replication kinetics between the two viruses (error bars, means ± s.e.m. of 8 independent experiments; P values are from paired Student’s t-tests).