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. Author manuscript; available in PMC: 2009 Sep 22.
Published in final edited form as: Nat Med. 2008 Jan 27;14(2):194–198. doi: 10.1038/nm1709

Figure 3.

Figure 3

C3d localization in the outer eye wall of the short-term immunization mice. (a) Frozen section showing the four tissue lamina in a differential interference contrast image. (b–e) Confocal images of Bruch's membrane in mice. (b) Confocal image of the outer eye wall from a CEP-MSA–immunized mouse showing the bright green fluorescence of the C3d-FITC probe along Bruch's membrane (arrow). (c) Image from another CEP-MSA–immunized mouse in an area where Bruch's membrane (arrow) labeling is not pronounced but intense C3d labeling is associated with two lysed RPE cells (on upper left of image) with profiles similar to those illustrated in the histology presented in Figure 2b–d. (d) Minor C3d labeling of Bruch's membrane (arrow) is present in a mouse immunized with non-adducted MSA. Differences in intensity and distribution of FITC fluorescence are present in b, c and d. (e) No C3d localization is evident in Bruch's membrane (arrow) in a naive mouse. Red fluorescence is the nuclear stain propidium iodide. Fluorescent intensity in an MSA immunized mouse (f) and a CEP-MSA-immunized mouse (g) was measured in the overlays shown. The long axis on the overlay represents 150 μm. Images in a–g are at identical magnification. (h) C3d fluorescence intensity from the groups specified. C3d immunofluorescence in the CEP-MSA group is five to six times higher than in the other groups.