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. Author manuscript; available in PMC: 2010 Aug 14.
Published in final edited form as: Mol Cell. 2009 Aug 14;35(3):280–290. doi: 10.1016/j.molcel.2009.06.010

Table 1.

Structural statistics for the major and minor S5a (196–306):K48-linked diubiquitin complexes.

Major Minor
Intermolecular NOE distance restraints (total) 46 36
 UIM1:distal ubiquitin 12 N/A
 UIM2:proximal ubiquitin 34 N/A
 UIM1:proximal ubiquitin N/A 12*
 UIM2:distal ubiquitin N/A 24
Ramachandran plot
 Most-favorable region (%) 79.5 79.0
 Additionally allowed region (%) 20.0 20.4
 Generously allowed region (%) 0.5 0.6
 Disallowed region (%) 0.0 0.0
r.m.s.d of backbone atoms to average structure (Å)
 UIM1: F206-M224; ubiquitin: M1-G76 1.15 ± 0.25 1.60 ± 0.85
 UIM2: P276-L295; ubiquitin: M1-G76 0.91 ± 0.28 0.76 ± 0.20
r.m.s.d of all heavy atoms to average structure (Å)
 UIM1: F206-M224; ubiquitin: M1-G76 1.47 ± 0.20 1.88 ± 0.73
 UIM2 P276-L295; ubiquitin: M1-G76 1.27 ± 0.27 1.13 ± 0.22
*

The distance restraints for UIM1:proximal ubiquitin used to calculate the structure of the minor S5a:K48-linked diubiquitin complex are derived from UIM1:distal ubiquitin intermolecular NOEs. See supplement for details.