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. 2009 Jul 19;18(20):3822–3831. doi: 10.1093/hmg/ddp325

Figure 2.

Figure 2.

Mutant H1 and H38 human L1-CAM protein both mediate wild-type level homophilic cell aggregation in Drosophila S2 cells. (A) Depicts a western blot of total S2 cell proteins that were isolated from the transfected S2 cell lines and subsequently tested for homophilic cell aggregation (B–F). (B–E) Show untransfected (B) or transfected S2 cells that express wild-type (B) or mutant (D—H1 and E—H38) human L1-CAM protein. The images show the presence of typical S2 cell aggregates for S2 cells expressing wild-type or mutant human L1-CAM. The size bar corresponds to 100 µm. (F) Depicts the quantification of homophilic S2 cell aggregation that is induced by the expression of the different human L1-CAM proteins. Shown are the results of five independent experiments. Compared with untransfected S2 cells P-values were calculated for wild-type L1-CAM transfected S2 cells as P = 1.3 × 10−4, for H1 transfected S2 cells as P = 0.5 × 10−4 and for H38 transfected S2 cells as P = 5.5 × 10−4.