Figure 5.

Neuroglian LOF phenotype in the adult Drosophila ocellar system and rescue by transgenic wild-type human L1-CAM. (A) Depicts the ocellar system of a wild-type fly. Neuronal cell bodies and axons were visualized by immunostaining with 22C10 antibody. (B) Shows the head of an nrg³ mutant fly that was incubated at non-permissive temperature during pupal development. The black arrowheads indicate a complete stalling phenotype of a BM axonal pathway, whereas the white arrowhead marks a partial stalling phenotype (not considered for data quantification). (C) Shows an example of the nrg LOF phenotype that was rescued by human L1-CAM expression, whereas (D) and (E) show a lack of rescue when H1 and H38 mutant human L1-CAM were expressed. The scale bar represents 100 µm.