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. Author manuscript; available in PMC: 2009 Sep 22.
Published in final edited form as: Parasite Immunol. 2009 Apr;31(4):210–219. doi: 10.1111/j.1365-3024.2009.01095.x

Figure 2.

Figure 2

Figure 2

Figure 2

Expression and functional activity of IsSMase. A, Purified recombinant IsSMase was resolved by 10% discontinuous sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and stained with Coomassie blue dye. Lane 1, molecular weight standard (kDa); Lane 2, flow-through; Lane 3, final wash; Lanes 4-8, eluates containing purified IsSMase (indicated by arrow). B, Functional activity of IsSMase was assessed by monitoring the rate of sphingomyelin hydrolysis at 37°C under standard conditions. C and D, effect of metal ions and pH on IsSMase activity. The sphingomyelinase activity of the IsSMase was recorded at multiple time points using excitation in the range of 530-560 nm and emission detection at 590 nm. Data plotted represent means ± SE (n=3). C, Activity of IsSMase at different pH.