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IsSMase enzymatic activity is not required to program CD4 T cell IL-4 expression. IL-4 expression potential in CD4 T cells exposed to bacterial SMase (10 U of Bacillus cereus SMase) and active vs denatured IsSMase. A, representative IFN-γ vs IL-4 intracellular staining plots and B, quantitative analysis. N=4 to 6 for each group.
