Fig. 1. Immunoblots showing the presence of SP-A and SP-D in whole lung surfactant (WLS) but not in calf lung surfactant extract (CLSE).
SP-A and SP-D were assessed in WLS and CLSE by immunoblotting as detailed in Materials and methods. Both surfactant preparations are also known to contain the hydrophobic lung surfactant apoproteins SP-B and SP-C (not tested for in the figure). Data shown are representative blots from 3 independent experiments on each surfactant preparation.