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. Author manuscript; available in PMC: 2010 Aug 18.
Published in final edited form as: Biochemistry. 2009 Aug 18;48(32):7584–7594. doi: 10.1021/bi9009264

Figure 5.

Figure 5

Membrane binding and insertion kinetics of the T-domain measured with LUV of specified lipid compositions (color-coded) at various pH (line coded). (A) Binding kinetics were followed by the change in FRET signal between donor-labeled T-domain and acceptor-labeled LUV. (B) Insertion kinetics were followed by changes in fluorescence intensity of environment-sensitive probe NBD attached to the center region of the membrane-insertion domain (Q369C-NBD). Membrane interactions were triggered at zero time by lowering the pH of the sample from pH 8 (no interaction, see Fig. 3) to the desired final value. Differences observed in the two types of kinetics reveal the insertion intermediate, which depends on pH and lipid composition.