FIGURE 10.

ATM activating agents do not induce high-level ULBP1 expression. A and B, FaDu cells were cultured with media (top panel) or with 0.3 U/L bleomycin (bottom panel) for 16 h. A, Cells were permeabilized and DNA content was measured with propidium iodide staining and flow cytometry. DNA content is shown on a linear scale, and cell cycle stages G₀/G₁ (open histogram), S (hatched), and G₂/M (cross-hatched) are indicated. B, Shown on a log scale is anti-ULBP1 mAb staining of FaDu cells treated with DMSO solvent alone (gray shading), bleomycin (thin line), or MG132 (thick line). C, FaDu cells were treated with aphidicolin (Aph) or hydroxyurea (HU) and cultured for 5 h before measurement of ULBP1 mRNA. Results are shown relative to the DMSO-treated control (Con). Show is a single experiment that is typical of at least two independent experiments. D, FaDu cells were given the indicated doses of gamma radiation, cultured for 5 h, and lysed. ULBP1 RNA was measured relative to GAPDH RNA and values were normalized to control nonirradiated cells. Shown are the means of two independent experiments and 95% confidence limits.