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. Author manuscript; available in PMC: 2009 Nov 15.
Published in final edited form as: J Immunol. 2009 May 15;182(10):6600–6609. doi: 10.4049/jimmunol.0801214

FIGURE 3.

FIGURE 3

Proteasome inhibitor drugs increase ULBP1 expression. A, FaDu cells were treated with DMSO solvent or the proteasome inhibitor as indicated for 5 h before cell lysis and analysis of ULBP1 mRNA. The relative level of mRNA was normalized to that of control DMSO-treated cells in the same experiment, indicated by the dotted line. Each proteasome inhibitor was tested at least twice. B, FaDu cells were treated with DMSO or with 3, 10, or 30 μM MG132 for 24 h and stained with mAb to MHC class I and to the NKG2D ligands, as indicated. Arrows and MG132 concentrations indicate treatment groups with staining intensity different from the DMSO-only control groups. Shown is one experiment out of at least 10 experiments with similar results, except that small shifts in MICA and ULBP2 staining were seen in a minority of experiments.