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. Author manuscript; available in PMC: 2009 Nov 15.
Published in final edited form as: J Immunol. 2009 May 15;182(10):6600–6609. doi: 10.4049/jimmunol.0801214

FIGURE 8.

FIGURE 8

The ULBP1 promoter is active in FaDu HNSCC cells and promoter activity is increased by proteasome inhibition. FaDu cells were transfected with the indicated pGL3-luciferase test plasmids as described in Materials and Methods. Promoters 0.5, 0.9, and 2.3 represent pGL3 plasmids containing 522-, 875-, and 2299-bp DNA, respectively, 5′ of the ULBP1 ATG translation start site. Values represent averages from independent tests of at least three different plasmid preparations (each measured in duplicate), with error bars representing 95% confidence limits. A, Cotransfected CMV-Renilla luciferase plasmid was used to control for transfection efficiency. “0” refers to the promoterless pGL3-basic plasmid. B, Cotransfected SV40-Renilla luciferase plasmid was used to control for transfection efficiency. Shown are results with the positive control SV40 promoter and test ULBP1 promoters in pGL3-luciferase. As described in Materials and Methods, 24 h after transfection, cells were cultured with DMSO or with MG132 for an additional 13 h before lysis. The histogram plots show the relative increase in activity over DMSO-stimulated controls.