Table 1. Summary of HHV-6 PCR, In Situ Hybridization, and Immunohistochemistry in Pediatric Brain Tumors and Control Non Tumor Brain.
| Pediatric Samples | HHV-6 PCR | HHV-6 ISH | HHV-6 IHC | ||
|---|---|---|---|---|---|
| MCP(%) | LTP(%) | HHV6 Subtype | MCP(%) | gp116/54/64 (%) | |
| TUMORS | |||||
| Paraffin-Embedded | 36/64 (56) | 43/64(67) | 31A 12B | 83/150 (55) | 50/124 (40) |
| Glioma | 27/41 (66) | 29/41 (71) | 23A 6B | 66/114 (58) | 41/88 (47) |
| Grade 1 | 10/18 (56) | 13/18 (72) | 30/46 (65) | 19/43 (44) | |
| Grade 2 | 2/2 (100) | 2/2 (100) | 16/29 (55) | 9/24 (38) | |
| Grade 3 | 6/7 (86) | 4/7 (57) | 13/24 (54) | 2/14 (14) | |
| Grade 4 | 9/14 (64) | 10/14 (71) | 7/15 (47) | 2/7 (29) | |
| Oligodendroglioma | 1/1 (100) | 1/1 (100) | 0A 2B | 5/10 (50) | 5/10 (50) |
| Ganglioglioma | N/A | N/A | N/A | 2/4 (50) | 2/4 (50) |
| Meningioma | 5/10 (50) | 7/10 (70) | 3A 4B | 5/10 (50) | 0/10 (0) |
| Medulloblastoma | 2/10 (20) | 4/10 (40) | 4A 0B | 4/10 (40) | 2/10 (20) |
| Germinoma | ½ (50) | 2/2 (100) | 1A 0B | ½ (50) | 0/2 (0) |
| Fresh Frozen | 32/56 (57) | 30/56 (54) | 12A 5B | N/A | N/A |
| Glioma | 14/24 (58) | 17/24 (71) | 12A 5B | N/A | N/A |
| Medulloblastoma | 14/24 (58) | 11/24 (46) | NP | N/A | N/A |
| Meningioma | 4/8 (50) | 2/8 (25) | NP | N/A | N/A |
| NON TUMOR CONTROLS | |||||
| Paraffin-Embedded Non-Tumor Brain | 7/32 (22) | 11/32 (34) | 4A 7B | 10/32 (31) | 6/32 (18) |
Abbreviations: HHV-6, Human Herpesvirus-6; MCP, Major Capsid Protein; LTP, Large Tegument Protein; ISH, In situ hybridization; IHC, Immunohistochemistry; NA not applicable; NP, not performed.