Table 1.
Primers used for amplification of sequences for each AcMNPV locus used and restriction enzyme site used for insertion of reporter and expression cassettes.
| Locus | Forward Primer | Reverse primer | Restriction enzymes used |
| ctx | CACTTGACTCGATTGCGCG | TATTTATTGTCTACATGAACACG | m*EcoRV |
| orf11 | GTTGCACCTTTGACGAAGCGG | TCACAATCCATAACACACAACAGG | m* EcoRV |
| egt | ATGTGCGACCATTGTTGGGC | GTTGTCACATCTGACTACTCC | pd |
| orf23 | AGAGTGCGTTAATCTGTACACC | ATCATAGGGTACAACACAGG | StuI/SfoI |
| v-fgf | CCGGCAAAATCAAAGCGAGC | GATTACACGTGACATTTACGATGG | m* EcoRV |
| 39k | CCTGGTAATTTTTGACCACG | CGCAGCAATTCCAGCGAGC | m* EcoRV |
| orf51 | AAATGACTAGACAAGAAATTGCC | AGTTGTACAAATCACAAATATAAAAG | BmgBI |
| gp37 | ATTGACGGGCCGTCGGCACG | CGATCATGCAAAAGTACATGC | m* EcoRV |
| iap2 | CCGCGGCTAAGCGTTAAACC | TTCGAATACGTGTGTCGTTTAATTTGC | BstBI/SacII |
| chiA | TAAACGCTCCGACTCTGTGG | CGAGGGCCGCGGCCAGTGGGTC | pd |
| pe | GCATTTTTCCAATGTGGTAGACG | CTTTAGCGGTTTCCAACGCC | m* EcoRV |
| odv-e18 | TCTCAAACACGGTGCCTGC | TCGTTGGTTTCAGTGACCAC | m* EcoRV |
| odv-e56 | CAACATGACGCCGCTGCCG | TTATCGAGGGGCCGTTGTTG | m* EcoRV |
m*EcoRV, indicates that an EcoRV site was engineered by site directed mutagenesis using the quickchange site directed muagenesis system (Stratagene) according to the manufacturer's directions. Pd indicates that a deletion was engineered by PCR. In the case of the egt locus this deletion removes the equivalent of nt 11622-12474 nt in the AcMNPV genome. For chiA the engineered deletion removes the chiA and cathepsin genes and is positioned nt 105461-107946 nt in the AcMNPV genome.