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. 2009 Sep 8;10:113. doi: 10.1186/1471-2202-10-113

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Copyright © 2009 Zhao et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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EndoG nuclear translocation preceded neuronal cell death following reoxygenation. (A-D) Confocal laser scanning microscope images of EndoG immunoreactivity (red) at control, EBSS, OGD/R6 h, OGD/R24 h, respectively. Co-staining with DAPI (blue) allowed the identification of nuclear translocation of EndoG. The white arrow indicates the nucleus with EndoG translocation but without pyknosis, and the white arrowhead indicates the pyknotic nucleus with EndoG translocation; (E) Percentage of damaged neurons and neurons displaying nuclear EndoG at different time points following reoxygenation. *p < 0.05 vs. control group, n = 9.