Figure 1.

Follistatin representative microscopic views of: mRNA expression (A, C, E, G, I) as detected by in situ hybridization (ISH); and protein expression (B, D, F, H, J) as identified by immunohistochemistry (IHC) for normal breast (NB), florid hyperplasia (FH), fibroadenoma (FIB), ductal carcinoma in situ (DCIS) and invasive ductal carcinoma (IDC). FST staining was detected in the epithelial cytoplasm (arrow) and nucleus (arrowhead) in the normal, benign and malignant breast tissue, with a stronger staining in the FIB peri-alveolar stromal cells. Negative controls were performed omitting anti-Dig antibody; Dig-labelled probe and anti-Dig antibody for ISH (K) and omitting the primary antibody for IHC (L). Bar 50 μm.