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. Author manuscript; available in PMC: 2010 Oct 2.
Published in final edited form as: Mutat Res. 2009 May 20;669(1-2):85–94. doi: 10.1016/j.mrfmmm.2009.05.004

Fig. 1.

Fig. 1

The target eGFP sequence has a single base mutation (bold) that codes for a premature stop codon and produces truncated, non-functional eGFP protein. Specific oligonucleotides (ODNs) were designed to bind the non-transcribed (NT) strand of the target eGFP gene (72NT and 72NT-PM) or the transcribed (T) strand (72T), (*) denotes phosphorothioate linkages. 72NT-PM is a perfect match oligonucleotide that complements the NT strand exactly, whereas, 72NT and 72T have one designed central mismatch to induce targeted gene alteration in the eGFP gene (bold). There were two nonspecific (NS) ODNs utilized throughout the experiments, neither bind the target eGFP gene.