Table IV.
Effect of sequence context on bypass efficiency of AP site in wild type yeast.
| Oligonucleotides anneal to leading strand; AP site bypass on lagging strand. | % bypassa (WT) | Oligonucleotides anneal to lagging strand; AP site bypass on leading strand. | % bypassb (WT) | % bypassb( rev1Δ) | % bypassb( rev3Δ) | % bypassb( rad30Δ) |
|---|---|---|---|---|---|---|
| COD(+7)-S1 | 7.3 ± 4.4 | NC(+7)-S1 | 11.5 ± 7.2 | 1.1 ± 0.4 | 0.8 ± 0.7 | 15.1 ± 4.3 |
| COD(+7)-S2 | 24.5 ± 2.3 | NC(+7)-S2 | 22.0 ± 12.6 | 1.8 ± 0.1 | 0.8 ± 0.5 | 18.9 ± 6.0 |
| COD(+7)-S3 | 44.6 ± 18.3 | NC(+7)-S3 | 21.7 ± 10.7 | 2.0 ± 0.5 | 1.5 ± 0.5 | 34.7 ± 13.1 |
| COD(+7)-S4 | 73.5 ± 11.4 | NC(+7)-S4 | 34.9 ± 3.9 | 11.4 ± 4.3 | 8.0 ± 5.9 | 36.5 ± 0.7 |
| COD(+7)-S4 | 18.3 ± 5.4c | ----- | --- | ---- | ---- | ---- |
| COD(+7)-S5 | 30.0 ± 6.2 | NC(+7)-S5 | 18.4 ± 0.4 | 7.5 ± 1.1 | 1.9 ± 0.5 | 28.8 ± 6.7 |
| COD(+7)-S6 | 33.0 ± 6.1 | NC(+7)-S6 | 14.5 ± 1.3 | 1.2 ± 1.0 | 0.6 ± 0.1 | 13.1 ± 3.7 |
| COD(+7)-S7 | 14.6 ± 3.3 | NC(+7)-S7 | 7.8 ± 1.0 | 1.1 ± 0.2 | 0.7 ± 0.3 | 6.4 ± 2.9 |
= average of three independent transformations ± sample standard deviation
= average of two independent transformations ± sample standard deviation
= average of two independent transformations ± sample standard deviation