Table V.
Effect of sequence context on bypass specificity of an AP site.
| oligonucleotide sequencea |
% nucleotide inserted (nob) |
|||
|---|---|---|---|---|
| dC |
dA |
dG |
dT |
|
| COD(+7)-S7 | 64(9) | 36(5) | - | - |
| 5′--GTCX | ||||
| --CAGSCGACCATTAA–5′ |
|
|
|
|
| COD(+7)-S6 | 56(10) | 44(8) | - | - |
| 5′--GTCTX | ||||
| --CAGASGACCATTAA-5′ |
|
|
|
|
| COD(+7)-S5 | 52(9) | 12(2) | - | 35(6) |
| 5′--GTCTGX | ||||
| --CAGACSACCATTAA-5′ |
|
|
|
|
| COD(+7)-S4 | 64(11) | 29(5) | 6(1) | 6(1) |
| 5′--GTCTGCX | ||||
| --CAGACGSCCATTAA-5′ |
|
|
|
|
| COD(+7)-S3 | 60(18) | 33(10) | 7(2) | - |
| 5′--GTCTGCTX | ||||
| --CAGACGASCATTAA-5′ |
|
|
|
|
| COD(+7)-S2 | 66 (14) | 34 (7) | - | - |
| 5′--GTCTGCTGX | ||||
| --CAGACGACSATTAA-5′ |
|
|
|
|
| COD(+7)-S1 | 56(18) | 38(12) | - | 6(2) |
| 5′--GTCTGCTGGX | ||||
| --CAGACGACCSTTAA-5′ | ||||
a
= S denotes the position of the AP site in the transforming ssOligo and X denotes the nucleotide inserted across from the AP site.
b
= number of transformants.