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. 2009 Jun 30;284(35):23251–23259. doi: 10.1074/jbc.M109.017384

FIGURE 5.

FIGURE 5.

Synaptic vesicles are present at α4β2 AChR/NRX-positive presynaptic terminals. A–D, neurons expressing α4β2 AChRs (red) and NRX (green) were coplated with tsA 201 cells expressing NLG and stained as described in the legend to Fig. 4, except that cells were alternatively costained for synapsin-1 (blue) instead of HA to detect synaptic vesicles at neuron-tsA 201 cell contact sites. Enhanced synapsin-1 clustering is observed at contact sites with NLG-expressing tsA 201 cells (arrows). Robust staining of presynaptic boutons with synapsin-1 Ab indicates presynaptic terminal maturation. The tsA201 cell is indicated by the dotted line. E–G, neurons expressing α4β2 AChRs (red) alone were coplated with tsA 201 cells expressing NLG (not immunostained). A vestigial synapse is shown (arrows). H–J, neurons expressing NRX (green) alone were coplated with tsA 201 cells expressing NLG (not immunostained). Single plane confocal images are shown. Scale bar, 10 μm.