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. 2009 Jun 30;284(35):23322–23330. doi: 10.1074/jbc.M109.037002

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© 2009 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 3. — A, sucrose gradient centrifugation of wild-type EL. After 5–20% sucrose gradient ultracentrifugation, samples were collected. Each fraction was tested for TG lipase activity (♦) and G6PDH activity (■). The percentage of sucrose was also measured in each fraction collected. B, molecular weight of wild-type EL determined by ultracentrifugation. The molecular standards used in this experiment were cytochrome c (12.5 kDa), ovalbumin (45 kDa), malate dehydrogenase (74 kDa), G6PDH (114 kDa), and catalase (240 kDa). After centrifugation (22 h at 200,000 × g at 4 °C), fractions were collected and assayed for enzyme activity and protein concentration. There is a linear relationship between the molecular weights of standard proteins and the sucrose percentage.