FIGURE 1.

Glutathionylation of Prx I. A, LC-MS analysis of Prx I glutathionylation. Glutathionylation was carried out by disulfide exchange with GSSG. The reaction mixtures containing 40 μm Prx I and 10 mm GSSG in Tris buffer, pH 7.4, were incubated at 4 °C for 18 h. The protein products were then subjected to reverse phase HPLC-MS analysis. B, Western blot analysis of Prx I glutathionylation. The time course of Prx I glutathionylation described in A was monitored by Western blot (WB) using an antibody specific for glutathione.