Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Identifying glutathionylated Srx as the reaction intermediate. Glutathionylated Prx I (C83S/C173S) mutant was incubated with Srx at 37 °C for 1 h in 50 mm Tris (pH 7.4) buffer, and the resulting mixture was subjected to LC-MS analysis. The extracted ion chromatogram shows each peak corresponding to each of the four different proteins: reduced Prx I (C83S/C173S), glutathionylated Prx I (C83S/C173S), reduced Srx, and glutathionylated Srx.
