TABLE 1.
Cysteine-containing peptides from control (upper lines) or glutathionylated (lower lines) Prx I (C52S) analyzed by LC-MS
Theoretical average m/z values of singly charged ions ([M + H]+) were calculated using GPMAW software (Lighthouse Data, Odense, Denmark).
| Residues | Retention time | Modification | Mass |
Sequence | |
|---|---|---|---|---|---|
| Theoretical | Observed | ||||
| min | m/z | ||||
| 37–66 | 26.7 | None | 3626.1 | 3625.3 | YVVFFFYPLDFTFVSPTEIIAFSDRAEEFK |
| 69–93 | 17.7 | 2 CIM | 2881.4 | 2881.9 | LNCQVIGASVDSHFCHLAWVNTPKK |
| 17.8 | 1 ACR/1 CIM | 2895.5 | 2896.0 | ||
| 169–190 | 13.9 | 1 CIM | 2406.2 | 2406.4 | HGEVCPAGWKPGSDTIKPDVQK |
| 14.0 | 1 ACR | 2420.2 | 2420.5 | ||