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. 2009 Jul 6;284(35):23375–23385. doi: 10.1074/jbc.M109.036848

FIGURE 1.

FIGURE 1.

Factor Xa-mediated propeptide processing of MMP-2 in rat aortic SMCs. A, serum-starved rat aortic SMCs were incubated in serum-free DMEM with or without 50 nm factor Xa for the indicated times, and the conditioned medium was analyzed by zymography. Conditioned medium from HT1080 cells was used as markers for MMP-2 and MMP-9 (n = 3). B, cells were pretreated with 1 μm rTAP or Pefablock TH for 30 min before incubation with factor Xa for 8 h. MMP-2 was concentrated from the conditioned medium using gelatin-Sepharose and analyzed by zymography and Western blotting (IB) with an anti-MMP-2 monoclonal antibody. The arrows indicate pro-MMP-2 and processed MMP-2 (n = 3). C, invasion assay for factor Xa-stimulated rat aortic SMCs. Note that non-stimulated cell invasion was slightly decreased in the presence of GM 6001. Data are represented as the mean and S.D. of n = 4 experiments. *, p < 0.05 factor Xa versus control (unstimulated cells); **, p < 0.05 factor Xa versus factor Xa plus inhibitors.