Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2009 Jun 29;284(35):23405–23414. doi: 10.1074/jbc.M109.026088

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2009 by The American Society for Biochemistry and Molecular Biology, Inc.

PMC Copyright notice

FIGURE 2. — Gene expression profiles of Cdk2ap1^−/− mESC embryoid bodies. The molecular effects of the deletion of Cdk2ap1 on the regulation of stem cell genes or differentiation-related genes were examined by quantitative reverse transcription-PCR analysis in differentiating embryoid bodies. A, WT (Cdk2ap1^+/+) or Cdk2ap1⁻^/⁻ mESCs were subjected to embryoid body formation and differentiation for 0, 5, and 10 days in culture. Total RNA was isolated after the indicated differentiation time period, and the level of Oct3/4 (p < 0.01) and Nanog (p < 0.04) was determined in biological duplicates. The statistical p value was determined by Student's t test by comparing values from two different genotypes for each differentiation time point. B, the level of differentiation-related genes, Hand1 (p < 0.03), Cdx2 (p < 0.005), Brachyury (p < 0.02), and Tpbpa (p < 0.01) was determined. Significant down-regulation of Hand1 and Cdx2 was observed in Cdk2ap1⁻^/⁻ mESC embryoid bodies, whereas Tpbpa was up-regulated in Cdk2ap1⁻^/⁻ mESC embryoid bodies. C, the effect of restoring Cdk2ap1 in Cdk2ap1⁻^/⁻ mESC embryoid bodies was examined at the molecular level. Selected clones stably expressing Cdk2ap1 were generated from Cdk2ap1⁻^/⁻ mESCs and subjected to differentiation as described above. The expression of Hand1 (p < 0.007), Cdx2 (p < 0.03), and Brachyury (p < 0.08) was up-regulated, whereas the expression of Tpbpa (p < 0.001) was down-regulated by restoring Cdk2ap1. The error bar represents S.E. The statistical p value was determined by comparing values against day 0. KO, knock-out.