FIGURE 5.

GRK2-mediated mGluR5 desensitization in striatal neurons. A, shown are representative immunoblots for mGluR5 and GRK2 protein expression in neurons infected with GFP, GRK2, GRK2-K220R, or GRK2-D110A adenovirus. 50 μg of cell lysate were used for each sample, and the samples were immunoblotted for the expression of GRK2, FLAG, and mGluR5 protein using anti-GRK2, anti-FLAG, and anti-mGluR5 antibodies. B, shown is mGluR5-stimulated inositol phosphate formation in response to either 1 or 10 μm DHPG for 5 min at 37 °C in striatal neurons infected with GFP, GRK2, GRK2-K220R, or GRK2-D110A adenovirus. Data represent the means ± S.E. of five independent experiments, normalized to DHPG-stimulated GFP-infected neurons maximum response. Asterisks indicate significant differences compared with GFP adenovirus-infected neurons at the corresponding agonist concentration (p < 0.05).