FIGURE 1.

Biochemical characterization of AtCSP3. A, schematic representation of the primary structure of AtCSP3 aligned with E. coli CspA and wheat WCSP1. B, complementation of cold sensitive growth of BX04 (ΔcspAΔcspBΔcspEΔcspG) with AtCSP3, cspA, and WCSP1. Overnight cultures of BX04/pINIII, BX04/AtCSP3, BX04/cspA, and BX04/WCSP1 were diluted to adjust cell density, spotted onto plates, and incubated at 37 °C for 1 day or 17 °C for 3 days. C, nucleic acid melting activity of AtCSP3. One pmol of annealed molecular beacon (a) was incubated with 300 pmol of GST (G), AtCSP3 (3), WCSP1 (W), CspA (A), or equal amount of the buffer (b). Fluorescence is expressed as a relative value against fluorescence of denatured beacon (d).