FIGURE 3.
Scanning electron micrographs of COD grown in solution in the presence of synthetic, low (a) and high (b) molecular weight aspartic acid-rich peptides at the indicated concentrations. a, increases in linear poly-Asp86–93 concentration (sequence from OPN bovine sequence), although not as effective as full-length OPN, induced a similar elongating effect on COD crystal morphology, increasing the {110} faces at the expense of the {101} faces. b, high molecular weight and poly-Asp (Sigma) was more potent in similarly generating rod-shaped crystal morphologies, with some additional “rounding” effect at the poles of the elongated crystals. Schematic insets show SHAPE software-derived renditions of the crystals used for face identification and c-axis determination (extension bars). Scale bars equal 5 μm.