FIGURE 4.

Comparison of the levels of enhanced GCase activity and protein in GD-1 and GD-2/3 fibroblasts treated with either ABX or IFG. A, N370S/N370S GD-1 fibroblasts treated with IFG (left panels) or ABX (right panels). B, F213I/L444P GD-2/3 fibroblasts treated with IFG (left panels) or ABX (right panels). In both N370S and F213I mutant cell lines, ABX generated comparable levels of enhancements of both GCase enzyme activity (black rectangles) and protein levels (open rectangles), derived from densitometry scans of the Western blot below the graphs, to those obtained at equivalent concentrations of IFG. In the Western blot panels, Mock represents the untreated cell lines. Bars represent S.E.