Skip to main content
. 2009 Jul 1;284(35):23540–23546. doi: 10.1074/jbc.M109.030072

FIGURE 1.

FIGURE 1.

AC5 associates with mAKAPβ in the heart. A, protein complexes were immunoprecipitated from adult rat heart extracts using mAKAP (VO54, VO56, or OR010), nesprin-1, or the corresponding preimmune (PI) sera. AC activity associated with the immunoprecipitates was measured in the presence of 50 nms + 100 μm forskolin (fsk). Significantly more AC activity was associated with the mAKAP and nesprin-1 antisera than the control PI sera. *, p < 0.05, n = 3. B, protein complexes were immunoprecipitated from rat neonatal ventricular myocyte extracts with VO56 mAKAP or PI sera. AC activity was assayed as in A. *, p < 0.04; n = 4. C, myocytes were infected with adenovirus expressing Myc-mAKAP-(585–1286), to inhibit endogenous mAKAPβ-nesprin-1α association, or β-gal control. AC activity in nesprin-1 or PI immunoprecipitates was measured by fsk-stimulated EIA. *, p < 0.05 compared with the other three conditions; representative of three experiments is shown. D, AC2, AC5, AC6, and/or Myc-tagged mAKAPβ were expressed in COS-7 cells. AC activity associated with Myc antibody immunoprecipitates was assayed as in A (left panel). The corresponding cell extracts were assayed for AC activity in the absence of phosphatase inhibitors (right panel). *, p < 0.05, n = 3. E, heart extracts were prepared from wild type (AC5+/+) or AC5 knock-out (AC5−/−) mice, and AC activity associated with mAKAP or PI immunoprecipitates was measured as in A. *, p < 0.05, n = 3.