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AC5 is inhibited by the mAKAPβ complex. A, AC activity associated with membranes prepared from COS-7 cells expressing AC5 and/or mAKAPβ was assayed in the presence of Gαs ± fsk. B, AC activity in membranes from COS-7 cells expressing the indicated proteins was assayed in the presence of Gαs and fsk. *, p < 0.05 and n = 3 for both A and B. C, representative Western blots of COS-7 membranes expressing the indicated proteins.
