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Estimated radii of recombinant LigBCen2, LigBCen2R, and LigBCen2NR
| Calculated mass | Mass spectroscopy | Expected RS from GPC calibration curve | RS from GPC | Expected Rh from DLS calibration curve | Rh from DLS | |
|---|---|---|---|---|---|---|
| Da | Da | nm | nm | nm | nm | |
| LigBCen2 | 18,130.97 | 18,131.60 | 2.05 | 1.95 | 2.04 | 2.07 |
| LigBCen2R | 13,473.76 | 13,416.81 | 1.67 | 2.07 | 1.75 | 1.71 |
| LigBCen2NR | 5,956.57 | 5,929.34 | 0.62 | 1.93 | 0.93 | 1.97 |
