TABLE 1.
The number of amide hydrogens in regions of GVIA-2 iPLA2 within a different range of rate constants
| Exchangeregion | Totalamides | PAPC/MAFP | Number of amide hydrogens indifferent exchange rate constants |
|||
|---|---|---|---|---|---|---|
| Fasta(K > 4.15 min−1) | Intermediatea(4.15 > K > 0.1 min−1) | Slowa(0.1 > K > 0.002 min−1) | Non-exchangeableb(K < 0.004 min−1) | |||
| 87–104 | 16 | − | 2.9 ± 0.2 | 3.7 ± 0.2 | 3.3 ± 0.2 | 6.1 ± 0.6 |
| + | 3.0 ± 0.1 | 3.7 ± 0.1 | 3.1 ± 0.1 | 6.2 ± 0.3 | ||
| 277–299 | 21 | − | 3.7 ± 0.1 | 2.0 ± 0.1 | 5.0 ± 0.1 | 10.3 ± 0.3 |
| + | 4.1 ± 0.3 | 2.0 ± 0.4 | 3.8 ± 0.4 | 11.1 ± 1.1 | ||
| 378–389 | 10 | − | 2.7 ± 0.1 | 2.2 ± 0.1 | 1.2 ± 0.1 | 3.9 ± 0.3 |
| + | 2.5 ± 0.4 | 3.6 ± 0.4 | 0 | 3.9 ± 0.8 | ||
| 631–655 | 23 | − | 7.7 ± 0.2 | 3.5 ± 0.2 | 4.6 ± 0.1 | 7.2 ± 0.5 |
| + | 6.4 ± 0.1 | 3.0 ± 0.2 | 0 | 13.6 ± 0.3 | ||
| 658–664 | 6 | − | 1.4 ± 0.1 | 0.8 ± 0.0 | 1.0 ± 0.0 | 2.8 ± 0.1 |
| + | 1.1 ± 0.1 | 0.6 ± 0.1 | 0.7 ± 0.1 | 3.6 ± 0.3 | ||
| 708–730 | 21 | − | 16.9 ± 0.2 | 0.3 ± 0.2 | 0 | 3.8 ± 0.4 |
| + | 3.2 ± 0.3 | 0 | 9.6 ± 0.4 | 8.2 ± 0.7 | ||
| 773–778 | 6 | − | 2.9 ± 0.1 | 1.2 ± 0.1 | 0 | 1.9 ± 0.2 |
| + | 2.5 ± 0.1 | 0.9 ± 0.1 | 0 | 2.6 ± 0.2 | ||
a From the curve fit of three-phase association or two-phase association in GraphPad Prism 4.0.
b Calculation by (Total exchangeable amides) − (Fast + intermediate + slow) = Non-exchangeable.