FIGURE 10.

BB NHE3 expression was decreased in NHERF3 knockdown Caco-2BBe cells. Caco-2BBe cells grown on filters were infected with 3HA-NHE3 adenovirus, fixed, and stained for NHE3 using anti-HA primary antibody. Confocal microscopy of serial sections (0.5 μm) of Caco-2BBe cells infected with empty vector control (A and B) or NHERF3 shRNA (C and D) stained with NHE3 and Hoescht 33342 (nuclear counterstain). Z-series images were reconstructed using MetaMorph Image Analysis software. BB NHE3 expression was significantly reduced in NHERF3 shRNA Caco-2BBe cells (C) as well as in cells (empty vector and NHERF3 shRNA) treated with 10 μm carbachol for 10 min (B and D). Results were obtained from three individual experiments.