FIGURE 8.

NHERF3 anchors NHE3 to the BB of Caco-2BBe cells and regulates basal and calcium-mediated inhibition of NHE3 activity. Using BCECF to measure initial rates of sodium/hydrogen exchange, basal NHE3 activity was significantly reduced in Caco-2BBe cells with stably knocked down NHERF3 compared with empty vector control. In addition, carbachol-mediated inhibition of NHE3 activity was abolished in NHERF3 shRNA Caco-2BBe cells. Bar graph summarizes the mean ± S.E. of nine experiments. p value is in comparison with control (analysis of variance).