FIGURE 9.

NHERF3 shRNA knockdown in Caco-2BBe cells. A, Western blot analysis of total cell lysates prepared from Caco-2BBe cells infected with lentivirus empty vector control or NHERF3 shRNA. Glyceraldehyde-3-phosphate dehydrogeanse (GAPDH) was used as internal control. B, NHERF3 protein expression was significantly reduced in Caco-2BBe cells stably knocked down for NHERF3 in complete media containing puromycin as selection marker. Bar graph summarizes the mean ± S.E. of four experiments. p value is in comparison with control (paired t test).