FIGURE 5.

Structural effects of metal binding to BdbD monitored by NMR. ¹H ¹⁵N-HSQC spectra of reduced sBdbD (200 μm) in 50 mm potassium phosphate, pH 7.0. The spectrum in green corresponds to the as-isolated Ca²⁺-bound form, and the spectrum in magenta resulted from Ca²⁺-depleted sBdbD. The spectrum in blue was obtained following the addition of an excess of Mg²⁺ ions to metal-depleted sBdbD.