TABLE 2.

Free energies of stabilization of oxidized and reduced sBdbD in the presence and absence of Ca²⁺ ions at 25 °C

sBdbD	Midpoint of transition (D0.5, mGdn-HCl)	Cooperativity	ΔGstab	ΔΔGox/reda
		m, kJ mol−1m−1	kJ mol−1	kJ mol−1
With Ca2+/reduced	3.60	8.5 ± 0.7	−30.4 ± 2.5	18.6
With Ca2+/oxidized	1.37	8.6 ± 1.1	−11.8 ± 3.5
Without Ca2+/reduced	3.28	6.4 ± 0.6	−20.9 ± 2.1	6.5
Without Ca2+/oxidized	1.35	10.7 ± 0.8	−14.4 ± 1.1

^aΔΔG_ox/red indicates the difference between the conformational stabilities of the oxidized and reduced proteins, i.e. ΔG_ox − ΔG_red.