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. 2009 Jun 26;284(35):23793–23805. doi: 10.1074/jbc.M109.025155

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© 2009 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 1. — Interaction of ErbB family members with Necl-2. Co-immunoprecipitation assay using HEK293 cells. Cells were co-transfected with various combinations of indicated plasmids and cultured in suspension. Each FLAG-tagged Necl family member was immunoprecipitated using the anti-FLAG mAb, and samples were subjected to Western blotting using the indicated Abs. A, assay for co-immunoprecipitation of ErbB family members with Necl-2. Aa, FLAG-Necl-2 and ErbB1; Ab, FLAG-Necl-2 and ErbB2; Ac, FLAG-Necl-2 and ErbB3; Ad, FLAG-Necl-2 and ErbB4. B, interaction of Necl-2 with ErbB3 through its extracellular region. Asterisks, full-length or mutants of Necl-2. C, assay for co-immunoprecipitation of ErbB3 with Necl family members. The results shown are representative of three independent experiments.