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No effect of Necl-2 on the HRG-induced formation of the ErbB3/ErbB2 heterodimer. HEK293 cells were co-transfected with various combinations of the indicated plasmids. ErbB2 and ErbB3 were immunoprecipitated using the anti-ErbB2 mAb and ErbB3 pAb, respectively, and samples were subjected to Western blotting using the indicated Abs. A, immunoprecipitation of ErbB2; B, immunoprecipitation of ErbB3. The results shown are representative of three independent experiments.
