FIGURE 2.

Sequence alignment of the Tom71/Tom70 family members. Program ClustalW was utilized to align the Tom71 sequences from S. cerevisiae (Sc Tom71) with that from C. albicans (Ca Tom71) and Tom70 from S. cerevisiae (Sc Tom70), H. sapiens (Hs Tom70), and D. melanogaster (Dm Tom70). The amino acid residues of yeast Tom71 are numbered above the alignment. The eleven TPR motifs (TPR1–TPR11) within Tom71 are labeled. Helices A0–A27 are labeled. The conserved residues involved in binding the Hsp70/Hsp90 C-terminal EEVD motifs are labeled with blue bars. The conserved residues involved in mediating the conformational changes generated by Hsp70/Hsp90 binding are marked with pink bars. The conserved hydrophobic residues forming the Tom70p preprotein-binding pocket among the family are marked with green bars.