Skip to main content
. 2009 Jul 6;284(35):23852–23859. doi: 10.1074/jbc.M109.023986

FIGURE 5.

FIGURE 5.

The cartoon drawing for the mechanism how Hsp70/Hsp90 prepares Tom70/Tom71 for preprotein loading. a, Tom70/Tom71 may exhibit two distinct states: the open and closed state. The Tom70/Tom71 molecule is shown in blue. The N- and C-terminal domains are labeled. The mitochondria outer membrane is shown in orange. b, the interactions between the Hsp70/Hsp90 C-terminal EEVD motif will lock the Tom70/Tom71 in the open state. The Hsp70/Hsp90 is shown in gold. The binding between Hsp70/Hsp90 and Tom71 could increase the volume of the preprotein-binding pocket. The complex formation might rotate the Tom71 C-terminal domain ∼20° back toward the closed state and therefore position the preprotein-binding pocket closer to the Hsp70/Hsp90. The Hsp70/Hsp90 EEVD motif is shown as a red arrow. The preprotein is shown as a green triangle. c, Hsp70/Hsp90 will then load the preprotein into the enlarged preprotein-binding pocket of Tom70/Tom71.