Figure 4. RT-PCR analysis of a pheromone-binding protein gene.

As previous results suggested that AtraPBP1 is expressed exclusively in male antennae, a more stringent RT-PCR analysis was performed and expression in other non-olfactory tissues was re-examined. AtraPBP1 was detected in male antennae (Ant), but not in legs (L), wings (W), thorax (Thx), or abdomen (Abd). A faint band, hardly seen in the figure, was observed in the original gel with female cDNA template thus suggesting basal expression in female antennae. RpL8 was used as a control gene.